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Specimen Collection
Phlebotomy Procedures Handling Considerations
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Handling Considerations
Universal Standard Precautions apply to all specimens of blood, serum, plasma,
blood products, vaginal secretions, semen, cerebrospinal fluid, synovial fluid, pleural
fluid, peritoneal fluid, pericardial fluid, amniotic fluid, and concentrated HIV or HBV
viruses. Any specimen of any type containing visible traces of blood should be
handled using Universal Precautions.
Serum and plasma are the most commonly used specimens in Chemistry procedures.
Serum is the intercellular fluid in blood without clotting factors. Serum is prepared
by centrifuging clotted blood. This means that whole blood specimens must be
allowed to clot before the serum can be separated. Twenty to thirty minutes is
usually required before centrifugation.
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