Phlebotomy Procedures
 
  Materials Required
  Tube Types And Order Of Draw
  Venipuncture And Skin Puncture
  Venipuncture Site Selection
  Venipuncture Sources Of Error
  Pediatric Patient Special Consideration
  Blood Culture Bottle Selection
  Blood Culture
  Adverse Patient Reaction
Handling Considerations
  Unable to Draw
  Patient Refusal
  Not Performing a Draw
  Drawing Drug Levels
  Non-blood Collection
 
Specimen Collection Phlebotomy Procedures Handling Considerations

Handling Considerations

Universal Standard Precautions apply to all specimens of blood, serum, plasma, blood products, vaginal secretions, semen, cerebrospinal fluid, synovial fluid, pleural fluid, peritoneal fluid, pericardial fluid, amniotic fluid, and concentrated HIV or HBV viruses. Any specimen of any type containing visible traces of blood should be handled using Universal Precautions.

Serum and plasma are the most commonly used specimens in Chemistry procedures. Serum is the intercellular fluid in blood without clotting factors. Serum is prepared by centrifuging clotted blood. This means that whole blood specimens must be allowed to clot before the serum can be separated. Twenty to thirty minutes is usually required before centrifugation.

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