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Specimen Collection 
Phlebotomy Procedures Venipuncture Using A Syringe
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A disposable syringe with a 21 to 27 gauge butterfly or needle may be used for
venipuncture on children less than 2 years old and patients with difficult veins (fragile,
thready, or "rolling" veins).
It is helpful to have assistance in emptying the drawn blood into tubes when performing
venipuncture with a syringe.
Venipuncture using a syringe is performed the same as routine venipuncture with the
following procedural changes:
- When assembling the equipment, place a sterile, sheathed needle or butterfly on the syringe.
- Proceed as in routine venipuncture.
- After sufficient blood has been drawn, remove the needle from patient’s arm and immediately
place a gauze pad on the arm and apply pressure to stop the bleeding.
- Transfer blood drawn into appropriate tubes as soon as possible. Delay may result in improper
coagulation.
- Tubes containing anticoagulants should be filled first using
the appropriate blood transfer device.
- After the patient is cared for, dispose of the syringe and needle as a unit in the
biohazardous sharps container.
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